For K. marxianus-treated coffee waste, the mixture was treated with K. marxianus mutant strain NRRL Y-50798 (Hughes et al. 2013) in a 30-L fermentation at 30 °C for 3 days at 100 rpm.
Upon completion, the reaction mixture was treated with additional chloroform (20 mL) and washed with distilled water (4 × 20 mL).
The digested nuclei mixture was treated with Proteinase K (Sigma-Aldrich, Missouri) and DNA was extracted using careful phenol chloroform extraction and ethanol/salt precipitation.
Resulting mixture was treated in specially designed vessels, under high pressure using microwave accelerated reaction system (MARS-5).
The impregnated black mixture was treated in H2/N2 atmosphere at a temperature varying from 180 to 500 °C.
The gas soot mixture was treated either as gray (using the effective absorption coefficient derived from total emissivity data or the Planck mean absorption coefficient) or as non-gray (using the weighed sum of gray gases model).
The mixture was treated with water and extracted with ethyl acetate.
The mixture was treated with 6 mL of TFA followed by 1 mL of triethylsilane (TES).
After the reaction, the radioactive mixture was treated as described for the conventional SA.
This mixture was treated with a saturated Na2CO3 solution and extracted with ethyl acetate.
Finally, the mixture was treated with 10 mL of hydrogen peroxide.
