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Exact(37)
Both pUC19 (lac+) and pYD1 (lac−) were shown to have the same efficacy of transformation and the mixture was transformed into E. coli, followed by selection on LB Agar containing ampicillin and X-gal.
The ligation mixture was transformed into E. coli DH5α and the transformants were selected for chlorampheniol resistance.
The ligation mixture was transformed into E. coli XL1-Blue and the transformants were selected on low salt LB agar plates containing 25 μg/mL Zeocin.
The ligation mixture was transformed into competent E. coli DH5α and the transformants were selected for chloramphenicol resistance.
Lastly, 10 ng of the ligation mixture was transformed into competent E. coli DH5α cells using the standard CaCl2 transformation protocol, and the cells were grown on LB solid medium supplemented with 50 μg/mL ampicillin.
The ligation reaction mixture was transformed into E. coli XL10 Gold.
Similar(23)
Finally, the digested mixture is transformed into competent cells to obtain the target clones.
Aliquots of the ligation mixture were transformed into competent E. coli TOP10 cells.
All ligation mixtures was transformed to ElectroMAX E.coli DH10b competent cells (Life technologies).
Following digestion with NcoI and BamHI, the library DNA was ligated into pET28a, the ligation mixtures were transformed into E. coli BL21, and transformants were screened as described above.
Reaction mixtures were transformed into E. coli strain DH5α as described above except transformants were selected on LB medium supplemented with kanamycin (50 μg/ml).
More suggestions(15)
solution was transformed
mixture was transferred
mixture was performed
mixture was consolidated
mixture was converted
blends was transformed
mixture was cultured
mixture was strained
mixture was monitored
mixture was set
mixture were transformed
mixture was poured
mixture was degassed
mixture was sampled
mixture was prepared
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