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The mixture was then assessed with 1 × PK reaction buffer.
This mixture was then fed to the fixed bed reactor.
The mixture was then shaken by hand.
The reaction mixture was then lyophilized.
This mixture was then passed over a QMA cartridge.
The mixture was then digested (about 90 min) until clear.
The mixture was then placed in the PPA cell.
The mixture was then serially diluted for the plate count.
The reaction mixture was then analyzed as above.
The crude reaction mixture was then purified by column chromatography.
The mixture was then sonicated for 30 minutes.
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