Sentence examples for mixture was thawed from inspiring English sources
The alkaline mixture was thawed on ice and neutralized by adding 2 μL of glacial acetic acid.
The mixture was thawed and filtered using Whatman number 1 filter paper to remove the bound tannin.
Then, the mixture was thawed and diluted in 3 ml of Hepes-KOH buffer to form proteo-liposomes. Liposomes were collected by ultracentrifugation at 100,000 rpm for 1 h at 4°C, using a Hitachi TLA100.3 rotor.
Aliquots of the SIS peptide mixture were thawed on wet ice, mixed briefly and spiked into peptide samples after enzymatic digestion and lyophilization and during solubilization just prior to solid-phase extraction.
Thereafter, reaction mixtures were thawed and subjected to SDS-PAGE electrophoresis and Western blot analysis.
Frozen protein mixtures were thawed, precipitated, and resuspended at 1 μg/μl in boiling 0.1 M Tris, 2% SDS (pH8.5).
The reaction mixtures were thawed and immediately applied to a 2.5 cm DE81 membrane (GE Healthcare, catalog no. 3658-325) under air pressure using a vacuum manifold (Millipore).
This stock solution of the standard mixture was aliquoted and stored at -80°C, and an aliquot was thawed on a daily basis for each ELISA microarray analyses.
Each serum sample of 100 µL was thawed, added to 70µLL of methanol-2-propanol mixture (80 20) and then vortex-mixed for 30 sec.
The mixture was then thawed and centrifuged for 30 min at 15,000 g.
