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For the in vivo studies the dry extract mixture was suspended in 0.9% saline (w·w− 1).
The bead mixture was suspended in CFPS reaction solution, emulsified into oil, and incubated.
Finally, the whole mixture was suspended with 2 mL of MQ water.
The lyophilized reaction mixture was suspended in aqueous HCl (1 N, 20 mL) and loaded on the column.
The XQLT mixture was suspended in distilled water to a fixed concentration for being orally administered to the mice through feeding needle and swallowing.
After a further centrifugation at 5,000 g for 20 min at 4°C, the pellet from the mixture was suspended once with 5 ml methanol and dried under nitrogen air flow.
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To prepare gels, HFH or Huh-7.5 cells and Bcl-2-HUVEC or a Bcl-2-HUVEC plus HGF-HUVEC mixture were suspended in rCI – human fibronectin (hFN) gels as previously described [12].
Thereafter, the mixtures were suspended in boiling water for 10 min to stop the reaction.
To prepare the PCR-mixture, each colony was suspended in DNase free water, which was subsequently heated, centrifuged and iced.
About 10 mg of freeze-dried faecal sample was suspended with mixture buffer [400 μl of 10% sodium dodecyl sulphate (SDS)/10 mM Tris HCl, 1 mM EDTA, pH 8.0 solution, 400 μl of phenol/chloroform/isoamyl alcohol (25 : 24 : 1), and 200 μl of 3 M sodium acetate].
A mixture of 9 0.5 g (1.85 mol) was suspended in absolute ethanol and anhydrous pyridine (0.3 ml) and carbon disulphide 0.5 mol (0.6 g) were added slowly.
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