Sentence examples for mixture was supplemented from inspiring English sources

Maximum lactic acid yield of 81 g/L was obtained when 15% (w/v) cassava bagasse treated with 12.5 mL/L enzyme mixture was supplemented with 7.5 g/L yeast extract and 3 g/L NH4Cl and inoculated with 3 × 1010 CFU/L of lactobacilli and incubated for 60 h at 37 °C as static culture.

The homogenized mixture was supplemented with a phosphatase inhibitor mixture (Sigma, P2850), protein kinase inhibitor H89 (Sigma, B1427) and a protease inhibitor cocktail (Sigma, P8340).

The final concentration of urea was then adjusted to 1 M and the mixture was supplemented with 4 µg sequencing-grade trypsin (Promega) for overnight digestion at 37°C.

When indicated, the reaction mixture was supplemented with 500 nM recombinant TXNL1 or TXNL1C34, 37S (after GST removal) that were either pre-reduced with 1 mM DTT (30 min, on ice) or pre-oxidized with 10 mM H2O2 (15 min, 25°C).

This mixture was supplemented with 1 unit of Taq DNA polymerase (Fermentas, Lithuania).

The initial rate of SGZ oxidation was highest when the reaction mixture was supplemented with 25% (v/v) DMSO.

Finally, we found that 100 μM Zn2+ inhibits the reaction when the mixture is supplemented with 10 μM Mn2+ for either l-Cys or BSH transferase activity.

Our results show that the levels of DSS1 adduct formation were significantly increased when the HeLa lysate/DSS1-biotin mixtures were supplemented with ATP (Fig. 2A; L5).

In all cases, the reaction mixtures were supplemented with β-glucosidase to avoid inhibition by product, maintaining a final enzyme concentration at 34 mM.

The hemolytic assay mixtures were supplemented with 2 mM DTT when 35S cysteine was used.

All reactions mixtures were supplemented with 0.05% Tween-20.