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The separation of the ITO co-element tin from the waste mixture was successively selective, and thereby minimizes the possibility of interference.
For purification, the mixture was successively washed with 5% HCl and deionized water for several times to completely remove residual salts and acids.
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After the completion of the reaction, the mixture was washed successively with 1 M HCl, saturated solution of sodium bicarbonate and saturated brine solution.
The reaction mixture was washed successively with water (2 × 20 mL), 10% NaHCO3 solution (2 × 15 mL), and water (2 × 15 mL).
The solid was redissolved in 80% trifluoroacetic acid and reacted for 90 min. The mixture was dialyzed successively against a 1 4 DMSO/water mixture and water.
The mixture was extracted successively with phenol saturated with TE [10 mM Tris HCl (pH 8.0), 1 mM EDTA] and chloroform.
After removal of HF and anisole in vacuo, the mixture was washed successively with anhydrous diethyl ether, then with acetic acid and the solution was diluted with methanol.
Afterwards, the obtained mixture was filtrated and the precipitate was successively washed by 95% ethanol, anhydrous ethanol and acetone.
Then, the mixture solution were successively added with 0.0225 mol of citric acid, 20 g of glucose, and 0.135 mol of acrylamide.
The HLC/n-HAp mixture was transferred into a mold, which was successively frozen at 4°C for 20 min, −20°C for 1 h, and −70°C for 3 h.
The obtained D0 of PAP was successively subtracted from the D0 spectrum of the resolved ternary mixture to get the D0 spectrum of binary mixture of CAF and PCT.
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