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The reaction mixture was stopped by addition of 5 ml of trichloroacetic acid (110 mM).
Subsequently, the heating of the mixture was stopped and the black-brown mixture was cooled down to room temperature (~2 h).
The reaction mixture was stopped by adding 10 μL of the stop solution into each well.
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After incubating the reaction mixture, the reaction was stopped by heating at 65°C.
After incubating lysates for 1h at 50°C with BAEE substrate mixture, the reaction was stopped by the addition of perchloric acid.
For analysing the CsLOX1 products, the reaction mixture of enzyme activity was stopped by adding 0.1 M HCl solution, n-hexane was then added to the mixture, and the products were extracted by shaking.
When the color of the mixture became blackish, the reaction was stopped and the product was dissolved in 1 mL of DI water.
After 24 h, aeration was stopped and the mixture was allowed to settle, then the supernatant was decanted and fresh synthetic wastewater was added to provide the same original volume.
Stirring was stopped and the mixture was heated at 70°C for 18 hours.
The reaction was stopped with a mixture of 5 mM Na2EDTA and 1% SDS and the DNA was separated on a 1.5% agarose gel.
At the end of the recordings, the sevoflurane delivery was stopped and a mixture of O2 and air was delivered via the mask to the monkey.
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