The resulting mixture was sonicated (2510 Bransonic sonicator, CT, USA) at 20 W for 12 h.
The mixture was sonicated using a horn sonicator (Bandelin GM 3200) for 30 min at room temperature in an ice bath to avoid overheating.
This mixture was sonicated using a microtip probe sonicator energy output of 55 W in a continuous mode (Soniweld Probe Sonicator, Imeco Ultrasonics, India) for a few minutes.
The mixture was sonicated (5 min) within a sonication bath, and vortexed (2 min) using a vortex mixer.
Then, the mixture was sonicated by a 14-mm-diameter probe sonicator, Hielscher UP400S (Tetlow, Germany).
The mixture was sonicated for 20 min at 500 W, 20 kHz, using Q 500 sonicator (Qsonica LLC, Newtown, CT).
Then the mixture was sonicated at 40 MHz at 25 °C for 5 min in a bath sonicator (Ultrawave Ltd).
The resulting mixture was sonicated for two cycles of three minutes each to generate an emulsion of pheomelanin microparticles.
The mixture was sonicated at 80 °C (Crest ultrasonic bath model 200 HT), under a 45 kHz frequency for 3 h.
The mixture was sonicated for 2 min.
The mixture was sonicated (10 min).
