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A fraction of each reaction mixture was saved as a control (total fraction).
5 µl of the PCR mixture was saved for nested PCR.
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After sonication, 100 mg of streptomycin sulfate was added, and the mixture was centrifuged, supernatant was saved, and ammonium sulfate was added to the final concentration of 0.56 g/mL.
The supernatant was saved.
Every tenth sample was saved, giving 9000 saved samples.
The supernatant (S20) was saved.
Substantial computation cost can then be saved.
Then, resources will be saved.
Time is saved, too.
Before further purification, 60 µL of mixture was removed and saved as a loading control.
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