Sentence examples for mixture was rotated from inspiring English sources

The mixture was rotated for 60 min at 4°C and centrifuged.

This mixture was rotated at 4°C for 2 h, either in the presence or absence of 10 µM celastrol.

The mixture was rotated for 30 minutes at 25°C and stored at 4°C until use.

To collect the immune complexes, appropriate Protein G Agarose mixture was added to each reaction mixture and the mixture was rotated for 2 hrs at 4°C.

Five µL of platelet suspension or Hanks buffer (buffer-control) were added to 45 µL of the IE suspension and the mixture was rotated for 30 min. Five hundred IEs were counted in triplicate for each sampleby fluorescence and direct light microscopy, with the adhesion of three or more IEs constituting a clump.

The mixture was rotated for 1 h at 4 °C.

Mixtures were rotated for 2 or 3 h at 37°C and the number of CFU was determined before and after incubation by plating dilutions on blood agar plates.

Protein G beads (GE Healthcare, Pittsburgh, PA) were added to all samples, and the mixtures were rotated overnight at 4°C.

Then, 25 μl of GammaBind G Sepharose (GE Healthcare) were added and the mixtures were rotated for an additional hour at 4°C.

One hundred microliters of the pre-cleared supernatant was mixed with an antibody (Supplementary Table 3) (Santa Cruz Biotechnology, Inc ., 25 μl Dynabeads protein G (Invitrogen, Thermo Fisher Scientific Inc ., and lysis buffer to make a 200 μl IP mixture that were rotated overnight at 4 °C.

Specifically, it was rotating.