In each group 20% of the cement content of the control mixture was removed, resulting in starting mixtures with 200, 240, 280, and 320 kg/m3 cement content.
After 5 h of incubation, bacmid Cellfectin mixture was removed and replaced with 2 ml of SF-900 II SFM 50 U ml−1 penicillin and 50 μg ml−1 streptomycin.
The remaining water from this mixture was removed azeotropically using acetonitrile.
Small aliquot of the reaction mixture was removed every 5 min and centrifuged at 20,000 rpm for 10 min to remove the adsorbent particles and measured the absorbance by UV Vis spectrophotometer.
10 mg of oil sample was mixed with 1 mL of toluene and then 2 μL of the solution mixture was removed diluted with 1 mL of toluene/methanol (1 1 v/v) solution.
Upon reaching room temperature, the reactor was degassed and a sample of the reaction mixture was removed, centrifuged, and the supernatant diluted and analyzed on a gas chromatograph coupled with a sulfur chemiluminescence detector (GC-SCD - Agilent 6C 6980 and SCD 335 (Agilent Technologies, Inc., Santa Clara, CA, USA)).
The transfection mixture was removed and replaced with complete medium.
The mixture was removed and 100 µl of fresh reaction buffer was added to cells.
Before further purification, 60 µL of mixture was removed and saved as a loading control.
The reducing mixture was removed and iodoacetamide in ammonium bicarbonate added and incubated for 20 min at 37°C.
Antibody mixture was removed by aspiration, sections were washed sequentially with buffer 1 (0.25% TX-100, 1x PBS) and buffer 2 (1x PBS).
