The emulsion was transferred dropwise to an aqueous solution of sodium cholate (V = 20 ml, 12 mM) and the mixture was probe sonicated at 18 W for 3 min to form w/o/w emulsion.
The emulsion was transferred dropwise to an aqueous solution of sodium cholate (V = 20 ml, 12 mM) and the mixture was probe sonicated at an amplitude of 60 W for 3 min to form w/o/w emulsion.
This single-phase mixture was probe sonicated for 30 sec at room temperature and then incubated overnight at 48 C.
In brief, the cell pellet was probe-sonicated in ice-cold sonication buffer (50 mM Tris-HCl, l0 mM MgCl2, 0.02% sodium azide, pH7.4) containing protease inhibitor cocktail.
The solutions were probe-sonicated (Soniprep 150) at power 10 for 3 seconds and incubated for 5 minutes at 37°C.
Both the composition and the spatial organization of the multilayered complex mixtures are probed.
The mixture was horn-sonicated for 5 min (VC 750, Sonics & Materials, Newtown, CT, USA), followed by a 10-min bath sonication NXP-10022, Kodo TecHwaseongesearch, Hwaseong, Korea).
After cooling down to room temperature, the mixture was ultra-sonicated mildly for a few minutes, the pH was tuned to 8.0 by NaOH in an ice bath, and we found a black flocculent deposit.
To extract nuclear proteins, the isolated nuclei were resuspended in NETN buffer (150 mM NaCl, 1 mM EDTA, 20 mM Tris-Cl, pH 8.0, 0.5% Nonidet P-40, 1 mM Na3VO4, 10 mM NaF, 1 mM phenylmethanesulfonyl fluoride, and 2 µg/ml aprotinin), and the mixture was sonicated briefly to aid nuclear lysis.
The mixture was sonicated briefly and incubated for 1 h with intermittent agitation.
