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The reaction mixture was placed on a shaker at 300 rpm and incubated at 37 °C for 10 min.
Approximately 3.0 g of the powder mixture was placed into a tungsten carbide ball mill jar, which was ball milled (SpexSamplePrep 8000 M) for 30 min.
The mixture was placed in cylindrical sample holder.
The mixture was placed in the dark for 72 h.
The mixture was placed into the wells of a 1.4%% agarose gel in TAE buffer.
The mixture was placed in a 10 mL seamless pressure vial.
The mixture was placed on a hot plate for 30 45 min to allow for oxidation.
The mixture was placed under reflux at 90 95 °C for 12 h.
The mixture was placed in an air-dry oven at 100 °C for 24 h.
The above mixture was placed in a shaker to get an even sample.
The mixture was placed in an autoclave and heated at 170 °C for 4 h.
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