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The mixture was overlaid with 2 ml of 30% sucrose and 1 ml of 5% sucrose.
The mixture was overlaid with 50 μl of mineral oil to prevent evaporation.
The reaction mixture was overlaid with 20 μl of mineral oil.
This mixture was overlaid on 6 ml of Histopaque (Sigma, Germany) and centrifuged for 10 min at 400 × g.
The mixture was overlaid on a plate containing LB solid medium and then incubated overnight at 37°C to produce the confluent lysis of the host cells.
This reaction mixture was overlaid with ethyl acetate, incubated overnight, and vortexed to extract hydrocarbons from the aqueous layer the next day.
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All the reaction mixtures were overlaid with 20 μl of sterile mineral oil.
One microlitre of premixed primers (0.05 μg μl−1) of TP and β-actin primers were added to aliquots of the master mix and reaction mixtures were overlaid with 50 μl of mineral oil.
A layer of a 1∶1 mixture of paraffin:silicon oil was overlaid onto the mother liquor present in the well.
A 1 mL reaction mixture in a test tube was overlaid with 1 mL of hexane immediately after addition of substrate, covered with aluminum foil, and incubated for 12 15 min at 30 °C.
To verify these findings, a second far Western assay was performed in which a blot of His6-tagged PGK or its truncations was overlaid with a Ciha-1 cells protein mixture (Figure 2B).
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