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In the negative controls, which never gave any significant staining, the Tunel reaction mixture was omitted.
As a negative control, the step using the TUNEL reaction mixture was omitted, and a nucleotide mixture in reaction buffer was used instead.
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As a response, new error rates called modified mean error were calculated for two-instrument mixtures using as reference the average amplitude of the partials, as shown in Table 4 the error values for the other mixtures were omitted because they have approximately the same behavior.
Calcium dependency of host NOS was investigated by incubating total protein extracts with both standard reaction mixtures (50 mM Tris-HCl, pH 7.4) and reaction mixtures from which CaCl2 was omitted.
Random primers were used and for each sample an equivalent mixture was prepared, from which Superscript III was omitted, to control for genomic DNA contamination.
SDS gel electrophoresis followed by autoradiography showed a prominent band at the position of the Adr1 DBD (18 kDa) that was absent if recombinant protein was omitted from the mixture (Figure 1A, B and Table 1 for yeast strains).
For experiments comparing positively and negatively supercoiled DNA, the negatively supercoiled plasmid was processed in a manner identical to that of the positively supercoiled molecules except that reverse gyrase was omitted from reaction mixtures.
As a negative control, 8-mer DNA was omitted from the mixtures, and the reaction was quenched after 5 min. After the reaction had been quenched, the automodified PARP-1 constructs were separated from the unmodified enzyme on an SDS PAGE gel and visualized by being stained with Coomassie Brilliant Blue.
RT– indicates that reverse-transcriptase was omitted from the reaction mixture.
For negative control, TdT was omitted from the reaction mixture.
This experiment was repeated with controls, where either purified SACOL1827 protein or core-RNAP was omitted from the reaction mixture.
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