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After shaking the mixture gently, the mixture was neutralised by the addition of (NaOH, 0.02N) until the first permanent pink colour was obtained.
The mixture was neutralised with 1 M hydrochloric acid followed by addition of 2 mL of 0.2 M phosphate buffer pH 7 [6].
At this point, 1 M HCl was added until the reaction mixture was neutralised.
After stirring for 2 h the reaction mixture was neutralised with 1 m aq.
Following cooling, the reaction mixture was neutralised by addition of 5 ml of 6% (w/v) sodium carbonate.
The mixture was neutralised with citric acid and 25 mM n-propanol added as the internal standard.
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Briefly, 30 g samples of litchi residue were treated with 120 mL of methanol/H2SO4 (90 10, v/v) at 85°C for 20 h, and the resulting mixtures were neutralised with 10 M sodium hydroxide before being extracted six times with ethyl acetate.
The mixture was allowed to cool and was neutralised with sat.
The acid was neutralised by adding 9 ml of 4-ethylmorpholine (Sigma), after which the mixture was dialyzed against phosphate buffered saline (PBS).
"One was neutralised and the other was cornered," he said.
Wales had no plan B once Gatlandball was neutralised.
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