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To this DNA solution, 200 μl TE-equilibrated (pH 7.5 – 8.0) Phenol solution (Roti-Phenol, Carl Roth, Karlsruhe, Germany) was added and the resulting mixture was manually mixed for 1 min.
The mixture was manually mixed for 90 s by a hand spatula.
The resulting mixture was manually shaken for 1 min.
Next, 200 μl of chloroform/isoamyl alcohol mix (24:1) were added, and the resulting mixture was manually shaken for 1 min.
Both FDB muscles from each mouse were incubated in collagenase solution at 37°C for 2 h, and the mixture was manually shaken every 30 min to improve digestion of the connective tissue.
Everyday, 52 mL sludge mixture was manually wasted from each reactor, and 52 mL pretreated and un-pretreated sludge was added to the respective reactors; AGS was fed back to reactors by sifting the wasted sludge through a sifter with aperture of 0.2 mm.
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The mixtures were manually turned at every 15th day to provide suitable aeration to the earthworms.
The mixtures were manually dropped into a calcium chloride solution of 3.5% (w/v) using a sterile syringe (bead size of 3 5 mm).
For all mortar mixtures, the mix proportion was manually selected to show a rheological behavior sensitive to the aggregate condition.
To maintain aerobic condition during composting, the mixture was turned manually every 3 days.
The mixture was then manually introduced into the activated fused silica capillary (75 μm i.d. × 360 μm o.d).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com