The mixture was loaded onto a gel filtration column (Superose6 10/300 GL, GE Healthcare).
Then, the mixture was loaded into a PTFE lined hydrothermal synthesis reactor.
The first-round PCR reaction mixture was loaded into the capillary and covered by oil.
The hydrogel mixture was loaded to a surface-treated PDMS mold using a pipette or a syringe.
The mixture was loaded into a Teflon-lined autoclave of 50 ml capacity under magnetic stirring and then tightly closed.
The resulting mixture was loaded into a 55 mL-Telfon-lined autoclave and maintained at 180 °C for 12 h.
The mixture was loaded onto a Superdex G200 column again to separate the Uch37-Rpn13C complex from excess Rpn13.
A polypyrrole (conductive)/dexamethasone (drug model) (PPy/Dex) mixture was loaded into the ERRs via a simple immersion process.
The crude mixture was loaded on a C-18 sep-Pak cartridge (Waters, Milford, MA, USA), primed with ethanol (5 mL) and water (10 mL).
The column was preconditioned by eluting with 10 ml PBS containing 0.5%% (v/w) BSA, and the reaction mixture was loaded onto the column.
For separation, equal volumes of amino acids to be separated were mixed and an aliquot (0.1 μL) of the resultant mixture was loaded onto the activated TLC plate.
