Sentence examples for mixture was layered from inspiring English sources

Then, the second-round PCR reaction mixture was layered on top of it.

After incubation, the mixture was layered over a Ficoll density gradient as described above to separate any unbound or non-internalized nanobeads from the ATM/ASC fraction, which was then plated for 24 hours and co-cultured as described above.

The reaction mixture was layered on top of a 10 mL 10 40% linear sucrose density gradient (in reaction buffer) and centrifuged at 78,000xg at 4°C in a SW41 rotor (Beckman) for 16 hours.

The reaction mixture was layered with 20 mL toluene.

After vortexing, the mixture was layered with mineral oil and incubated for 16 hr at 54°C on water bath.

This mixture was layered carefully over 15 mL of Ficoll-Paque in a 50 mL falcon tube.

Reaction mixtures were layered over a 10%40%% linear sucrose gradient (50 mmol/L Tris-HCl [pH 7.4], 500 mmol/L KCl, 2.5 mmol/L MgCl2, 1 mmol/L DTT) and centrifuged at 30,000 rpm in an SW32 rotor (Beckman Coulter) at 4°C for 7 h.

qualPCR mixtures were layered with 50 µl of mineral oil and subjected to one cycle of 94°C for 3 min, followed by 30 cycles of 1 min of denaturation at 94°C, 1 min of annealing at 62°C, and 2 min of extension at 72°C, and a final extension step of 7 min at 72°C.

This mixture (30 ml) was layered over 20 ml of Lymphoprep and centrifuged at 800 × g for 30 min.

The reaction mixture was carefully layered onto a 100 μl cushion of buffer S with 15% glycerol in a TLA100 centrifuge tube.

The mixture was then layered on top of the 0.6 ml of 2.3 M sucrose/buffer TKM in a SW41 centrifuge tube (Beckman, CA, USA) and centrifuged at 10,000 g for 1 h.