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Then the mixture was irradiated by 16-W ultraviolet irradiation (Philips, Amsterdam, The Netherlands) at room temperature.
The mixture was irradiated by the UV lamp with an irradiance of 1.1 mW/cm2.
The mixture was irradiated by an UV lamp (350 to 400 nm wavelength range) with an irradiance of 1.1 mwhich (which simulates the UV irradiance of the sun on the Earth).
The mixture was irradiated with a tunable, pulsed laser at wavelengths in the actinic UV range of λ = 300 330 nm (AC does not absorb significantly for λ > 330 nm).
Measurement of single-turnover kinetics for RNA cleavage revealed that in the SBA constructs E11 and E13, five- to sixfold higher catalytic rates were obtained when the reaction mixture was irradiated with visible light (favouring trans-Az) as compared to ultraviolet light (which promotes cis-Az), consistent with trans-Az in these constructs stabilizing the enzyme substrate complex.
An experiment where a H2O CO2 NH3 = 1 1 1 ice mixture was irradiated using the ultraviolet/extreme ultraviolet (UV/EUV) light provided by a synchrotron beam in the broad 4 20 eV (62 310 nm) range at 16 K is presented here.
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After this time, the various mixtures were irradiated by UV light (40 W cm−2).
In the actual reaction setup, the reaction mixtures were irradiated under normal solar light with an intensity of 80,000 100,000 lx at ca. 25 °C for different durations.
The mixtures were irradiated ultrasonically for 30 min. Then 100 mL of the acidic aqueous solution of APS (containing APS 6.80 g and conc. HCl 1.00 mL) was added dropwise into the colloidal mixture within 30 min with magnetic stirring in an ice-water bath.
Briefly, BamHI (3,000 U/µl) (without BSA) (New England Biolabs, Ipswich, Massachusetts, USA) was diluted in the specified bacterial ultrafiltrate to 0.54 U/µl (Figure 1B) or diluted in the various reagent-mixtures to 3.6 U/µl (Figure 3A, 4C, 4D, S3B, S3C); typically, 200 µl of the BamHI mixtures were irradiated aerobically.
After adding riboflavin, the reaction mixtures were irradiated (300 µmol m−2 s−1, provided by three 23 W Osram DULUX PRO compact fluorescent lamps) for 10 min at 25 °C, and the absorbance at 560 nm was measured using a non-irradiated reaction mixture as a blank.
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