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The mixture was inserted using a micropipette (100 μL) in situ after injury and just before the suture.
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Ten frozen pellets of 10 μL each of this mixture were inserted in the polarizer, along with ten frozen pellets of 10 μL each containing 3 M ascorbate (Sigma Aldrich) in D2O to scavenge the radicals after dissolution.
Five frozen beads of 10 μL each of this mixture were inserted with five frozen beads of 10 μL each containing 3 m ascorbate in D2O to scavenge the radicals after dissolution.
In this method, a matrix solid-phase dispersion (MSPD) column including a suitable mixture of polar sorbents was inserted in the mobile phase pathway, and while the interfering compounds were retained, the analytes were eluted and entered into the analytical column.
When the tip of the dipstick strip was inserted into the mixture, PCR amplicon-blue latex hybrids moved up through the strip by capillary action and were captured by tag-complement oligonucleotide fixed as test line on the strip, resulting in accumulation of blue latex microspheres to induce a visible blue test line.
The temperature of the reaction mixture was controlled by placing the cuvette holder in a water bath, and the temperature was measured using a thermocouple probe that was inserted in the reaction mixture.
A glass capillary (inner diameter 0.9 mm) containing 50 μL of this mixture and closed by epoxy glue [29] was inserted into the gel.
The mixture of Si and SiO 2 powder (molar ration 1 1) was placed over the AAO template, and a piece of carbon cloth was inserted between the template and the mixture powder.
A 1" diameter quartz tube was inserted inside an isothermal furnace, and the source mixture was kept in a quartz boat inside this tubular reactor.
A laryngeal mask was inserted, and anesthesia was maintained with isoflurane (2 to 3%) in a 1 1 oxygen/air mixture.
Then the laparoscope was inserted.
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