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The pH of the mixture was initially adjusted to 5 using 4 M KOH.
A control mixture was initially produced with ponded ash, Portland cement, Class F fly ash, and water.
As we realize that pH assumes a vital part in metal ion binding, thus, pH of the reaction mixture was initially adjusted using either HCl or NaOH (1 M).
The mixture was initially incubated at 95°C for 10 min, followed by 40 cycles of 95°C for 15 seconds and 60°C for 60 seconds.
Each mutation-specific primer mixture was initially evaluated against both cloned lab-generated and patient-derived mutant virus sequences that were serially diluted 10-fold in backgrounds of wildtype sequence plasmids.
The reaction mixture was initially heated to 95°C for 3 minutes, then 65 cycles of 95°C for 15 seconds, 64°C for 10 seconds, and 72°C for 45 seconds.
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We assumed isothermal conditions (temperature=1,425 K) and that the CO2 H2O mixture is initially saturated at 200 MPa.
The fluid mixtures were initially lumped with a clustering technique.
Using 3PrimePCR thermocycler (Techne, UK), reaction mixtures were initially incubated at 94 °C for 2 min, followed by 35 cycles of amplification (94 °C for 30 s, 42 °C for 50 s, and 72 °C for 35 s), followed by final extension at 72 °C for 5 min.
Reaction mixtures were initially incubated for 30 s at 95°C, followed by 35 cycles of 5 s at 95°C, 30 s at 55°C, and 20 s at 72°C.
The reaction mixtures were initially desalted to remove reagents and salts.
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