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Exact(54)
After this, the mixture was immediately cooled down to have the intact cell structure broken down.
The mixture was immediately homogenized using Ultra Turrax T-25 homogenizer for 5 min.
Chloroform was then evaporated under a gentle stream of nitrogen, and the resulting mixture was immediately diluted with 50 ml of distilled water.
The final mixture was immediately deposited on the transducer surfaces using the Eppendorf microsampler (with variable volume 0.1 to 2.5 μL) till complete coverage of the working surfaces.
Then, the mixture was incubated for 10 min, diluted 2.5 times in the same buffer, and a 5-μl portion of the mixture was immediately placed onto mica.
In the next step, a colloidal silica was added dropwise to the solution under stirring and the mixture was immediately placed in an oil bath equipped with a magnetic stirrer and temperature controller.
Similar(6)
The reaction mixtures were immediately placed in bright sunlight and observed for change in color of the solutions.
Reaction mixtures were immediately filtered on a nitrocellulose membrane using a slot-blot device.
The mixtures were immediately centrifuged at 3,000 × g for 15 min and the supernatants were stored at −20°C until analyzed for LPS and VFA detection.
The mixtures were immediately homogenized on ice using a Polytron homogenizer (Kinematica, Switzerland) at 15 000 rpm for six cycles, each with 10-s bursts and 5-s pause times.
When the preincubation was complete, media was removed from the 96-well plate harboring the TZM-bl cells, and the EGCG/virus mixtures were immediately added in triplicate (50 μl per well) to 10 TZM-bl cells seeded in collagen-coated 96-well microplates.
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