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The mixture was hydrolyzed by heating for 5 min at 100 °C.
After completion of the reaction, the mixture was hydrolyzed.
The mixture was hydrolyzed with 5 mL 5% aqueous HCl (5 mL).
The mixture was hydrolyzed with HCl (1 M) at 133°C for 4 h20.
The mixture was hydrolyzed by addition of saturated NaHCO3 solution (10 mL) and concentrated under reduced pressure.
The resulting mixture was hydrolyzed with trifluoroacetate (0.2 mL) in dichloromethane (0.2 mL) at room temperature for 10 min to deprotect BOC.
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A mixture of 3,4,5-trimethoxyphenylacetic acid (1), 3-hydroxy-4-methoxybenzaldehyde (2), triethylamine, and acetic anhydride was heated at reflux at 140°C to give intermediate 3. The reaction mixture was then hydrolyzed using sodium hydroxide, and the cinnamic acid analogue 4 was precipitated from the solution following acidification with concentrated hydrochloric acid.
Briefly, 4-O-pivaloyl-L-dopa (PharmaSynth As, Tartu, Estonia) was dissolved in acetic acid and acetyl [18F]hypofluorite was bubbled through the solution at 130°C for 10 min. The reaction mixture was then hydrolyzed in 6 M HCl at room temperature for 3 min. The product was purified using high-performance liquid chromatography (HPLC) on a COSMOSIL 5C18-MS-II column (20 mm (i.d).
However, when a phospholipase is present in the reaction mixture, phospholipids are hydrolyzed and more useful oil is available for lipase methyl ester formation.
The substrate was hydrolyzed with Trichoderma reesei cellulase in commercial mixtures.
Tetraethyl orthosilicate was hydrolyzed with magnetic NPs as seeds in an ethanol/water mixture.
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