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Liver tissue portions (100 mg from the right side of the ventral lobe) were added to 3 mL ice-cold methanol chloroform (2 1) and the mixture was homogenised for 1 min in a homogeniser (HeidolphDiax, Schwabach, Germany).
The mixture was homogenised at a speed of 22,000 rpm for 3 min using a rotor stator homogeniser (IKA Labortechnik homogeniser, Selangor, Malaysia).
The mixture was homogenised by swirling manually.
The mixture was homogenised using homogenizer for 5 min in an ice bath.
The mixture was homogenised for 1 min, boiled for 2 min and stirred for 4 h at room temperature (28 30 °C) using a magnetic stirrer (Analisis, Mumbai, India).
The mixture was homogenised and incubated for 12 h at 6 °C.
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Pour the warm cream into the funnel at the top of the processor and pulse once or twice more, just enough so the mixture is homogenised.
The mixtures were homogenised and incubated for 1 h at RT. 500 µl of each mixture was injected by intraperitoneal route into each mouse of a group of 4. The mice were observed every day for 96 h.
A total of 500 μl TRIzol reagent was added to frozen cell pellets or tissue samples; these mixtures were homogenised, and 100 μl chloroform was added.
Hepatopancreas (25 g) was homogenised with the mixture of chloroform: methanol: distilled water mixture (50 100 32.4, v/v/v) at the speed of 9500 rpm using an IKA Labortechnik homogeniser (Selangor, Malaysia) for 2 min at 4°C.
"It was homogenised, Hollywoodised.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com