Sentence examples for mixture was held from inspiring English sources

A binding buffer suspension (500 μL) was added to the cells followed by 5 μL of the FITC-Annexin V mix, and the resulting mixture was held at 4°C for 20 min.

Tetraethyl orthosilicate was added to the mixture, and the stirring was continued for another 20 h, at 308 K; later the mixture was held at 373 K for 48 h.

After adding SDS to a final concentration of 1% v/v, the mixture was held 2 h at 25°C in the dark, and proteins were precipitated with TCA [trichloroacetic acid], 10% v/v for 1 h.

The reaction mixture was held at 37°C for 40 min. The reaction was quenched by adding 5 mg/ml NaBH4 in 100 mM K2HPO4, 100 mM KCl, 30% tetrahydrofuran, resulting in the liberation of β-ketoacyl groups from their respective thioesters as acyl-1,3-diols.

The mixture was held at 37°C for 50 min.

The mixture was held in electric oven with designated temperature for 20 min.

The 18F mixture is held in place by capillary forces until it settles.

PCR amplification was conducted as follows: PCR mixtures were held at 94°C for 4 min, followed by 35 40 cycles of 94°C for 30 s, 55-60°C 55-60°Cs and 72°C for 30s.

The virus-serum mixtures are held overnight at 4°C and then inoculated into ten roller-tube cultures followed by 1 ml of a suspension containing 105 cells in growth medium.

The surface pressure, equal to the difference between the surface tension of the gas water interface of pure water and that of the interface under study, was measured as the monolayer films of the peptides and peptide/lipid mixtures were held at a constant area and exposure to gas-phase ozone.

Two and a half microliters of the PI mix was then added to the mixture, and the resulting cell suspension was held at 4°C in the absence of light for 15 min.