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During the doughy stage of polymerization, the mixture was hand-molded into a hemispheric shape around the sterilized head, according to the diameter of the acetabular cup.
The material was mixed until a doughy paste was obtained, and the gentamicin-PMMA-MMA mixture was hand-rolled into beads.
The mixture was shaken by hand for 30 s and then centrifuged for 5 min.
The mixture was prepared via hand mixing with agathic mortar and pestle of powders of individually synthesized CER network and amino-MMT.
The mixture was shaken by hand for 30 s, vortexed for 15 s, and centrifuged at 2200g, 4 °C for 6 min.
About 60 to 80 g of the mixture was compacted by hand in a stainless steel cup (4.0 cm in diameter) and placed in a high-pressure reaction vessel for combustion (Figure 1).
The mixture was shaken by hand for 1 min and then kept in dark for 5 min. After the addition of 75 mL of distilled water, the mixture was titrated against sodium thiosulfate (0.002 mol/L) until the yellow color almost disappeared.
The mixture was ground by hand on ice until only fibrous white insoluble material remained.
Tissue was chopped into a semifine slurry, and the resulting mixture was swirled by hand until the liquid obtained a light-green tinge.
One volume of 5% bleach (equal to the sputum volume) was added to each specimen and the mixture was shaken by hand continuously for 10 minutes.
Kelsey and Alex told me the mixture was ready and handed me a curdled, lukewarm tube of plaster; I looked down at it and immediately went limp.
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