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Hybridisation mixture was finalized by adding TE-3-buffer to 84 μl together with 17 μl 20x SSC and 3 μl 10% SDS followed by denaturing at 100°C for 2 minutes, renaturing at room temperature for 15 minutes and spinning at 13000 rpm for 10 minutes.
Similar(59)
Finally, the gradient procedure was finalized, as described in "HPLC Analysis".
The deal was finalized last week.
Within hours, Gore's approval was finalized.
By Thanksgiving of 2007, the sale was finalized.
That process was finalized last month.
The sale was finalized in 2006.
(The divorce was finalized in 2012).
The divorce was finalized last January.
The deal was finalized the following year.
The withdrawal was finalized in August 2009.
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