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The lipid mixture was dissolved in the solution of chloroform and methanol (3:1).
After cooling the mixture was dissolved in chloroform and filtered off.
The reaction mixture was dissolved in diethyl ether and washed with water excessively.
The mixture was dissolved with 10 mL 0.10 mol/L H2SO4 solution and diluted to 25 mL with water.
After 5 days, the reaction mixture was dissolved in toluene and passed through an aluminum oxide column to remove the copper catalyst and then precipitated in n-hexane.
The above redox mixture was dissolved in 2 ml of deionized water and then it is taken into a muffle furnace at 500 °C.
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When such a mixture is dissolved, the large content of ammonium ion renders the solution slightly acidic, thus preventing oxidation of iron (II) [36] as well as the hydrolysis of aluminium and other hydrated cations.
The probe mixture is dissolved in hybridization mixture, denatured, cooled, and mounted with 22 × 60 mm coverslip.
Approximately 300 ng of probe from this mixture were dissolved in 10.5 μl Hybrizol VII (Q-BIOgene) and 0.75 μg each of human and manatee Cot-1 DNA.
Stir until the gelatin mixture is dissolved.
20 mg of solid sodium alginate (or of the lyophilized degelling mixtures) was dissolved in 1 mL of D2O and analyzed in a Bruker NMR Avance 400 MHz instrument.
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