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The reaction mixture was directly microfiltered after the addition of acidified water without previous settling.
That is, N,N′-bis trimethylsilyl -substituted m-pheN,N′-bis trimethylsilyl -substitutedthaloyl chloride iN,N′-bis trimethylsilyl -substitutedamide, and the resulting mixture was directly cast into films.
The mixture was directly submitted to flash chromatography on silica gel with pentane EtOAc (3/1) as eluent affording product 9 (65% yield for (P,P -trans-L2; 90% yield for (M,M)-cis-L2; 85% yield for (P,P -trans-L2
Upon cooling to room temperature and determination of the labeling yield using Radio-TLC, the labeling mixture was directly diluted with assay buffer to a final peptide concentration of 20 nM and used as such for the subsequent in vitro studies.
The reaction mixture was directly analyzed by reverse-phase HPLC on a Phenomenex Luna column (5 µm, 25 × 1 cm), eluted with isocratic 0.1%% formic acid in CH3CN/H2O (15:85) at a flow rate of 2.5 mL/min for 5 min, followed by 0.1%% formic acid in CH3CN/H2O (25:75) for 30 min, and a wash with 100%% CH3CN for 10 min.
This cell lysate mixture was directly poured to a 50 ml syringe attached to glass syringe filters.
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As can be seen in (7), the relative amplitudes to be assigned to the partials in the mixture are directly proportional to the warped correlations of the partial with respect to the reference partials.
After DpnI digestion of the mixture of the amplified vector and insert to eliminate the DNA templates used in PCR reactions, the mixture is directly transformed into competent E. coli cells to obtain the desired clones.
A wide range of oxygen and methane mixtures was directly processed without dilution or explosion at ambient temperature because the microscale plasma reactor removes excess heat generated by partial oxidation, thereby maintaining a reaction field at temperatures near room temperature.
From previous studies, we conclude that grass crude protein (CP) content in alfalfa-grass mixtures is directly proportional to the percent alfalfa in the mixed stand.
Quenched digestion mixtures were directly desalted and analyzed by MALDI-TOF as indicated above.
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