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Thereupon the mixture was cooled to room temperature and then added portionwise to saturated aqueous NaHCO3.
Then, the reaction mixture was cooled to room temperature and precipitated with 50 ml of water.
The mixture was cooled to room temperature, acidified with 2 M HCl and the methanol was removed in vacuo.
Thereafter, the mixture was cooled and filtered.
The mixture was cooled to 4°C.
Then, the mixture was cooled to ambient temperature.
After this step, the mixture was cooled to room temperature.
The mixture was cooled in ice cold water.
The resulting yellow mixture was cooled to room temperature.
The mixture was cooled to room temperature and centrifuged.
Thirdly, the mixture was cooled down to 0 °C and stirred for 24 h.
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