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The mixture was cool to the room temperature, and yellow solution was filtered, and solvent was removed to yield dry extract.
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Thereafter, the mixture was cooled and filtered.
The mixture was cooled to 4°C.
After this step, the mixture was cooled to room temperature.
Then, the mixture was cooled to ambient temperature.
The mixture was cooled in ice cold water.
The resulting yellow mixture was cooled to room temperature.
The mixture was cooled to room temperature and centrifuged.
Then the reaction mixture was cooled, and the obtained suspension was filtered off.
Thirdly, the mixture was cooled down to 0 °C and stirred for 24 h.
The mixture was cooled to room temperature, and the product isolated by filtration.
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