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The gas mixture was contained in a gas bag which was connected to the interface.
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We recorded how many of the mixture parameters were contained in their respective confidence intervals.
We note that over 90%% of the genes are contained in mixture components Comp.3 and Comp.7.
It is difficult to explain why the mixtures of various differentiation phases are contained within MSC spheroids.
Next, a protein binding mixture was prepared containing 200 nM TF in PBS-2% (wt/v) BSA, 51.3 ng/μl salmon tested DNA (Sigma) and 50 μM zinc acetate.
Next, a second mixture was made containing 4 µL of First Strand Synthesis Buffer, 1 µL of dithiothreitol, 1 µL of RNAse inhibitor and 0.5 µL of Superscript III RT.
A stock mixture was prepared containing 15% w/w C18 0 and 85% w/w C18 1.
50 μl reaction mixture was used containing 1× buffer, 20 μM primers, 2.4 μM dNTPs and 1.5 U Taq polymerase.
A reaction mixture was made containing 8 μL of the PCR product, 1 μL SA buffer (F. Hoffmann-La Roche Ltd Group, Basel, Switzerland), and 1 μL (10 U) of each restriction enzyme.
A reagent mixture was prepared containing of β-carotene (0.2 mg/ml in chloroform), linoleic acid (0.02 ml) and Tween 80 (0.2 ml).
A mixture was prepared containing 1.275 ml of buffer with SYBR Green (2× Master Mix SABiosciences RT2 qPCR), 1.173 ml RNAse-free H2O and 102 μl of the cDNA sample.
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