Sentence examples for mixture was constituted from inspiring English sources
Using seven purified enzymes (cellobiohydrolases, Cel7A, Cel6A, Cel6B; endoglucanases, Cel7B, Cel12A, Cel61A; and β-glucosidase) from Trichoderma viride T 100-14 mutant strain, a multi-enzyme mixture was constituted after screening and optimization.
The following mixture was constituted using the iScript One-Step RT-PCR kit for SYBR Green assay (Biorad, Nazareth, Belgium): p289-290 primers (300 nM each), 0.5 μl enzyme mix, 12.5 μl reaction buffer, BSA (400 ng/μl final concentration), 2 μl of RNA extraction, ultrapure RNAse free H2O (Invitrogen, Carlsbad, USA) to 25 μl.
A total of 25 μL PCR reaction mixture was constituted with KAPA 2G fast hot start ready mix (1X containing KAPA2G fast hot start DNA polymerase, KAPA 2G fast hot start PCR buffer, 0.2 mM dNTP each, and 1.5 mM MgCl2), with 15 pmol of Bg3/Bg4 primers and 12.5 pmol of TR3/TR4 primers and additional 1 mM MgCl2.
The mobile phase was constituted by a mixture of methanol:water 60 40, containing 2 · 10 mol L - 1 phosphoric acid).
Shivpuri was constituted a municipality in 1904.
It was constituted a municipality in 1867.
Mandla was constituted a municipality in 1867.
Panipat was constituted a municipality in 1867.
Karnal was constituted a municipality in 1867.
Chandpur was constituted a municipality in 1897.
Rangpur was constituted a municipality in 1869.
