The assay mixture was consisted of 1.99 ml 50 mM sodium phosphate buffer (pH 7.0) supplemented with 0.1 μM EDTA, 10 mM guaiacol and 15 mM H2O2 and 100 μl of the enzyme extract in a total volume of 2 ml.
PCR reaction mixture was consisted of 1 μl cDNA, 10 μl pre-mix, 1 μl evergreen fluorescence dye (SolGent, Korea), and 500 nM of each primer (except for 18S reran that 250nM of each primer were used).
qRT-PCR reaction mixture was consist of the KAPA SYMR FAST qPCR Kit (KAPA BIOSYSTEMS) and 2 μl four times diluted cDNA reaction mixture in a final volume 20 μl with 200 nM of the gene specific primers as listed in Additional file 2. PCR reaction was performed with iCycler iQ (BIO-RAD) and the cycle as follows, denaturation at 95°C for 1 min, annealing and polymerization at 58°C for 20 seconds.
The reaction mixture were consist of 0.5 ml enzyme solution and 0.5 ml of 1% colloidal chitosan in 1 ml McIlvaine buffer at the indicated pH.
Heterogeneous asphalt mixtures are consisted of very irregular aggregates, asphalt matrix and a small amount of air voids.
However, very limited information has been reported concerning the modification of a matrix, such as a screen-printed carbon electrode (SPCE) which is consisted of a mixture of graphite and pasting binder, using plasma.
Distinct band with molecular weight of ∼25 kDa was observed in the SDS-PAGE of enterokinase digested mixture, which was consisted with the theoretical molecular weight of CfLec-1.
50 μl of reaction mixture was prepared consisting of 50 mM Tris HCl (pH 7.4), 10 mM MnCl2, 5 mM bis-pNPP to which was added 50 μl of the protein sample.
A 6-μl mixture was prepared consisting of 3.5 μl sterile dH2O, 2 μl of restriction enzyme buffer, and 0.5 μl restriction enzymes (HaeIII and MseI).
Therefore, a gas mixture was used consisting of 5% oxygen and 95% nitrogen to decrease the oxygen supply while keeping the aeration rate at 0.013 vvm.
To calibrate the system, a calibration gas mixture was used consisting of acetaldehyde, acetone, isoprene, benzene, toluene, xylene and α-pinene (covering molecular weights from 32 to 136 amu), each in a concentration of 1 ppmv (parts per million volume, ±5 %) (Linde, Dieren, The Netherlands).
