Sentence examples for mixture was completed from inspiring English sources

The fast release of GA from the physical mixture was completed within 7 min at pH 7.4 (Fig. 5a).

After incubation and centrifugation, this mixture was completed with 2 µl of 5× first strand buffer, 1 µl of DTT at 20 µM, 1 µl of dNTPs (10 mM each) and 1 µl of MMLV reverse transcriptase (Clontech, Mountain View, CA, USA).

The DNA mixture was completed to 1 μg with pBluescript as carrier DNA.

The reaction mixture was completed by adding 0.25 μL of 30 μmol/L of each forward and reverse primer (Integrated DNA Technologies) and 10 μL of DNA from each sample tested.

Reaction mixtures were completed by addition of 50 150  μg membrane protein to a final reactive volume of 50  μl and shaken gently.

After addition was completed, the mixture was stirred for additional 30 min to remove all HCl.

After the addition was completed, the mixture was stirred for additional 30 min to remove all HCl.

After the stirring was completed, the mixture was centrifuged at 4,500 rpm to separate the capped gold nanoparticles.

After the digestion was completed, the mixture was allowed to cool for 30 min and the TRN was determined by with a Kjeltec auto 2300 analyser.

Once the reaction was completed, the mixture was diluted with water (30 mL) and passed through a Waters Sep-Pak tC18 Plus Long SPE cartridge (WAT036800).

After the reaction was completed, the mixture was cooled down to room temperature, and the particles were redispersed in MQ water and sonicated for 1 h.