Sentence examples for mixture was cleared from inspiring English sources

The reconstitution mixture was cleared by centrifugation before applied to size-exclusion chromatography (Superpose-6 10/300 GL, GE Healthcare) in buffer containing 20 mmol/L Hepes, pH 7.4, 150 mmol/L NaCl, 2 mmol/L DTT).

Bio-beads were then removed and the reconstitution mixture was cleared by centrifugation before subsequent separation on a Superose 6 column (GE Heath Care) in buffer (20 mmol/L HEPES, 150 mmol/L NaCl, pH 7.4, 5 mmol/L CaCl2).

The mixtures were cleared by centrifugation, incubated with coloring solution, and absorbance at 570 nm was obtained using Appliskan (Thermo Electron Corp., Madison, WI, USA).

The solution was heated until the mixture was clear, and then kept aside to cool slowly overnight at ambient temperature.

Isopropanol was added when slurry was formed and it was heated up to 80 °C until the mixture was clear, then it was taken to the oven to be dried at 100 °C.

Once sugar is completely dissolved and mixture is clear, remove from heat and allow to cool.

Briefly, 24 hr after transfection, the cells were lysed on ice for 30 min in lysis buffer (50 mM Tris/HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 10% glycerol) with protease inhibitor mixture, and the lysates were cleared by centrifugation.

The refolding reaction mixture was then cleared by centrifugation to remove misfolded species.

Larvae were cleared in 1 1 benzylalcohol:benzylbenzoate mixture and mounted on glass slides.

For this 5 × 10 pLUM, pLB or a 1 1 pLUM pLB mixture was injected into cleared mammary fat pads of ovx'd mice without hormone supplementation and grown into tumors for 6 weeks.

At this end, the mixture was totally clear forming a slight yellow color.