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To each sample 200 µl KOH (50%, w/v) and 3 ml methanol were added, the mixture was capped with argon, vortexed and incubated at 80°C for 1 h, followed by lipid extraction using 3 ml of water and 5 ml of HPLC grade hexane (Sigma-Aldrich).
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The reaction mixture was allowed to equilibrate for several minutes, after which it was capped and a baseline was measured for 50 s.
Unfortunately, existing models of the climate are not accurate enough to resolve this dispute with the precision that policymakers would like.If greenhouse-gas emissions are to be capped, however, a mixture of political will and technological fixes will be needed.
Following coupling, the resin was drained under vacuum and washed and free sites were capped with a mixture of Ac2O∶ NMP∶ pyridine.
The tubes were capped and the mixture vigorously shaken for 10 s.
The beads were capped with a mixture of 10 mL 0.2 M phenoxyacetic anhydride in dry THF and 10 mL of 0.2 M 1-methyl imidazole and 0.2 M 2,4,6-trimethylpyridine in dry THF for 10 min at room temperature.
After the coupling step, unreacted amino groups were capped by treatment with a mixture of acetic anhydride (20%) in DMF followed by a washing step with DMF.
The remaining free resin hydroxyl groups were capped by treating the resin with a mixture of acetic anhydride/pyridine (2∶3) and shaking for 2 h.
The leaking well is capped.
Rail fares would be capped.
Expenses are capped at 1.0%.
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