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A double volume of a phosphate-buffered saline solution was then added, and the mixture was briefly vortexed.
The mixture was briefly vortexed and heated at 100 °C in a water bath for 1 h and terminated the reaction in an ice bath.
After 5 min, 450 µl CHCl3:MeOH HCl (50∶100∶1, v/v) was added and the mixture was briefly mixed and snap-frozen in liquid nitrogen.
Following incubation for 10 min at 30°C in a shaking incubator, the reaction mixture was briefly centrifuged and the supernatant transferred to a 96-well microtiter plate.
150 µl of LoTE (3 mM Tris.HCl pH 7.5, 0.2 mM EDTA)/7.5 M ammonium acetate (5∶1 ratio) was added and the mixture was briefly vortexed and incubated at 65°C for 2 hours.
The mixture was briefly vortexed and chilled on ice for 10 minutes.
Similar(44)
The methods for determining transport numbers of individual ionic species in mixtures are briefly mentioned.
The role of edge-flames in the failure of upward rising methane/air flames in sublimit mixtures is briefly discussed.
The mixtures were briefly vortexed and incubated at room temperature for 30 min for complexes formation.
In Section 2, the modeling and parameter estimation of Gaussian mixtures is briefly described.
The reaction mixtures were briefly vortexed and then incubated at 30C° for 30 min with occasional mixing.
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