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The mixture was boiled at 100o C for 15 min.
The mixture was boiled for about 20 min on a sand bath.
After soaking, the mixture was boiled for 10 min and filtered.
The mixture was boiled on a hot plate for 10 min and then cooled under running water.
The mixture was boiled in 90 °C for 15 min, after it was refrigerated in cool water bath (0 °C).
Then, 0.75 ml of oleylamine was rapidly injected, and the resulting mixture was boiled for another 2 h.
0.2 M NaOH was fully mixed with cell pellet, and the mixture was boiled for 12 min to breakdown the cells and release proteins.
The distillate mixture was boiled for 1 h under air reflux in water bath, cooled, volume made upto 250 ml and then filtered.
The mixture was boiled in water bath for 15 min and cooled in ice, and its absorbance was measured at 532 nm.
To the sample, 5 mL of 65%% HNO3 was added, and then the mixture was boiled gently for 30 45 min.
The mixture was boiled for 5 min and filtered first through sterile muslin cloth and Whatman filter paper before finally decanting it.
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