Sentence examples for mixture was assembled from inspiring English sources

Briefly, the strand-displacement reaction mixture was assembled in 30 µl volume with 30 mM Hepes, pH 7.5, 30 mM KCl, 8.0 mM MgCl2, 1.0 mM DTT, 100 µg/ml BSA, 0.01% (v/v) Nonidet P-40, 2.5 nM of 32P-labeled 63-mer F-DNA substrate, 2 nM of APE, 5 nM of Pol-β and 0-125 µM of NSC-124854.

For PCR amplification, a 25 μl reaction mixture was assembled containing 200 ng of genomic DNA, 10 pmol of primer, 4 mM MgCl2, 0.2 mM dNTPs, PCR buffer and 1.25 units of Taq DNA polymerase (Invitrogen).

The TaqMan PCR reaction mixture was assembled from a 2× ddPCR Mastermix (Bio-Rad), 20× primer, and probes (final concentrations of 900 and 250 nM, respectively) and template (variable volume) in a final volume of 20 μL.

A 30 µl reaction mixture was assembled consisting of second round cDNA diluted 1 1000, dNTP mix (0.2 m m in final concentration), forward and reverse primers (0.2 µM each in final concentration), Platinum Taq PCR buffer, 1.5 m m MgCl2 and 1.8 U of Platinum Taq DNA polymerase (Invitrogen life technologies, Carlsbad, CA, USA).

Duplicate triplex reaction mixtures were assembled.

Reaction mixtures were assembled on ice in a total volume of 30 µL containing 50% (v/v) reticulocyte lysate, 500 µg/mL cyclohexmide, 200 µM sparsomycin, 2 mM DTT, 100 mM KCl, 0.5 mM MgOAc2, and any additional test compounds (e.g. edeine), and pre-incubated at 30°C for 5 min to allow the drugs to interact with the translational machinery.

Amplification reaction mixtures were assembled as described, with PCR buffer modified to contain only 1 mM magnesium chloride.

The library was assembled in 174 positional scanning mixtures (58 + 58 + 58), each of which contained 3364 compounds (1 × 58 × 58).

Yet another "emergency taskforce" was assembled.

An inquisition was assembled.

The force was assembled in November 1939.