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The genomic DNA mixture was amplified using ILPs.
The mixture was amplified by polymerase chain reaction (PCR).
Ten-fold diluted ligation mixture was amplified with adapter-specific primer MseI-N (5′-GATGAGTCCTGAGTAAN-3′) (25 μM).
The resulting cDNA mixture was amplified by PCR using one of the 20 TMR-labeled arbitrary anchored primers (M13r-ARP).
The PCR mixture was amplified in a DNA thermal cycler with the specifications described in materials and methods.
A 2.5 μl aliquot of the 40 μl ligation mixture was amplified in a 50 μl PCR reaction.
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In addition, the use of plasmid DNA as a stuffer serves as a good quality control for the amplification product, giving a good indication that the amplified mixture is amplified without a bias, before engaging in further expensive analytical procedures.
The resulting DNA mixture is amplified with external oligonucleotides that act as suppression adapters.
Two microlitres of cDNA from the RT reaction mixture were amplified in a 25 μL reaction volume containing 10 pmol of each primer, 200 μM dNTP, in buffer containing 1.5 mM MgCl2 and 5 U of Taq polymerase (Fermentas).
Each chromosome mixtures was amplified, barcoded and sequenced separately (IlluminaHiSeq).
The cDNA mixtures were amplified by PCR using LRRC25-specific forward (5′-GGTGCCTGCTTCTTGGACTTG-3′) and reverse (5′-AGGTCGATGTCCTTGTAGTTGATGT-3′) primers and 2× Taq PCR StarMix (GenStar, China).
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