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After denaturation (80 °C for 7 minutes), the probe mixture was added to the denatured chromosome.
Breading for the crab cake mixture was added with a light hand.
Finally, the remaining mixture was added.
Briefly, 300 mg of the surfactant mixture was added to 300 mg of cinnamon oil.
One hundred microlitres LDH reaction mixture was added into each well.
The synthetic HM mixture was added to each 100-L aquarium and vigorously stirred.
The reaction mixture was added into water, and then extracted by EtOAc for three times.
To the stirred mixture was added t-BuONO (0.46 g, 4.5 mmol, 1.5 equiv).
Then, TUNEL reaction mixture was added to the cells at room temperature for 1 h.
Then, 50 µL of TUNEL reaction mixture was added per sample containing approximately 106 cells.
This mixture was added to aldehyde 3 in DMSO (3 ml) and stirred for 30 minutes.
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