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Resolution of the racemic mixture was achieved with chiral HPLC technique.
In addition, the highest creep stiffness, with an enhancement of about 25% relative to the control mixture, was achieved with the 5% treated WCO mixture to resist permanent deformation.
Good chiral separation of the racemic mixture was achieved in less than 4 min using a fused-silica capillary and as background electrolyte (BGE) a phosphate buffer solution (20 mM, pH 7) containing 0.15% (w/v) of CM-γ-CD as chiral selector.
At each addition, homogeneity of the mixture was achieved by magnetically stirring the mixture until the PVT cell pressure was stabilized at the set value.
Next, 2.5 g (8.9 × 10−3 mol) of oleic acid (Sigma-Aldrich, 99 + %) was added dropwise into the obtained complex, and the resulting solution was gently stirred for 2 h at room temperature until the complete homogeneity of the reaction mixture was achieved.
Recently, using the same technique, purification of soy peptides from a complex mixture was achieved [ 126].
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Under optimal conditions, total elimination of the original compounds and 94% TOC removal of the mixture were achieved in 5 and 194 min, respectively.
Sensitivities of 103 and 104 target copies/μl initial ligation mixture were achieved, depending on the PRI-lock probe.
Combine all ingredients thoroughly until a creamy mixture is achieved.
In the process of mixing, equal consistence of all concrete mixtures was achieved.
Separation of complex peptide mixtures was achieved by using reversed-phase chromatography.
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