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Systematic gradient resolution experiments were conducted for this mixture using reversed phase chromatography.
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The reaction mixture was then diluted with 100 ml 5% Glacial acetic acid (GAA), and loaded onto pre-activated C-18 columns (Burdick and Jackson, Muskegon, MI) to purify the conjugate using reversed phase chromatography.
Fold changes were computed using reversed VST transformations averaged for each strain.
The stability of CAST was monitored throughout the study using reversed phase high performance liquid chromatography.
The supernatant was analyzed using reversed phase HPLC and electrochemical detection.
MGEScan-non-LTR returned no results using reversed silkworm genomic sequence.
The solution was concentrated using reversed phase SPE cartridges (see below).
Peptides were enriched from the digestion mixture using reversed-phase (RP) C18 Sep-Pak cartridges (Waters) and the eluate was dried to completion in a vacuum centrifuge.
Strain mixture using muslin cloth.
Puree the mixture using a handheld mixer.
Dampen the mixture using a spray bottle.
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