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We have developed a technique for removing PCR error products, unincorporated primers and dNTPs from a PCR product mixture using immobilized metal affinity chromatography (IMAC).
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Table 1 shows results of batch acidogenesis of sucrose and raffinose mixtures using immobilized cells on porous γ-alumina.
In order to identify novel interaction partners of p66Shc contributing to its complex regulation and localization, we performed an in vivo pull down experiment using immobilized p66CH2CB (a mixture of dimeric and tetrameric state) and cleared mitochondrial lysate.
This resulted in producing a mixture of the amine and aldehyde forms in solution when accelerated reactions were used, such as using immobilized enzyme or microwave reactors.
These problems could be overcome using immobilized yeast.
This added to the significant reduction of the cost of purification of ethanol when immobilized cells are used, points to the convenience of using immobilized biocatalyst.
The antiserum was subsequently affinity-purified using immobilized recombinant Sp2.
2D arrays using immobilized proteins may also be used.
His6-ubiquitin conjugates were purified using immobilized Co2+ resin.
The immune sera were affinity purified using immobilized peptides.
Thus, the aim of this work was to evaluate the ability of pure as well as mixed actinobacteria cultures isolated from different contaminated environments, to degrade a pentachlorophenol and chlorpyrifos mixture, using free and immobilized microbial cells.
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