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Surface cleaning is performed under an Ar-1 vol.% N2 gas mixture using either a dielectric barrier discharge (DBD) process or a microwave post-discharge process at atmospheric pressure.
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The plates were then surface treated by pre-oxidation and nitridation in N2 4H2 based gas mixtures using either a conventional furnace or a short-cycle quartz lamp infrared heating system.
The proportion of wild ancestry fish declined over time in both smolt and adult datasets using either mixture (geneclass2) or admixture (structure) proportions (Fig. 1; P < 0.05 all analyses, Table S2).
As we realize that pH assumes a vital part in metal ion binding, thus, pH of the reaction mixture was initially adjusted using either HCl or NaOH (1 M).
The pH of the mixture was adjusted to 8.0 using either aqueous NaOH or HCl as required and the mixture was allowed to stir for 20 min. A solution of 21 b (0.500 g, 0.89 mmol) in 5: 1 mLOH/CH2Cl2 (90 mL) was then added in portions over 5 min. The solution was allowed to stir for 16 h at RT. On completion, the reaction mixture was diluted with CH2Cl2 (150 mL) and washed with brine (2×100 mL).
The pH of the mixture was adjusted to 8.0 using either aqueous NaOH or HCl as required and the mixture was allowed to stir for 20 min. A solution of 21 a (0.500 g, 0.88 mmol) in 5 1 mLOH/CH2Cl2 (90 mL) was then added in portions over 5 min. The solution was allowed to stir for 16 h at RT. On completion, the reaction mixture was diluted with CH2Cl2 (100 mL) and washed with brine (2×100 mL).
Separation of gas liquid mixture, which is achieved by using either large gravity separators or compact separators is a common and vital operation in the petroleum industry.
The pH of the mixture was adjusted to 8.0 using either aqueous 50%% NaOH aq).
The liagtion mixtures were used either directly for PCR (referred from now as Lig-PCR) or for transformation of chemically competent One Shot® Top10 E.coli cells (Invitrogen, Carlsbad, CA, USA).
Risk analysts evaluate information regarding the mode of action associated with individual mixture components and then use either 'dose addition' or 'response addition' to predict effects.
2. Strain the mixture using the back of a large spoon.
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