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The highest temperature and the highest yields of liquid bio-oil and gas were obtained with the 75:25 mixture treated with 800 W total power using both magnetrons.
Figure 3 shows the GPC profiles of the reaction mixture treated with or without PahZ1KP-2 for 24 h.
The reaction was stopped by incubation at 70°C for 10 min, and the reaction mixture treated with RNaseH (BioLabs) according to the manufacturer's instructions before dilution with 600 μL of sterile de-ionized water.
An aliquot (200 μl) was taken from the assay mixture, treated with 2 ml of 3,5-dinitrosalicylic acid (DNS) solution (40 mM DNS, 400 mM NaOH, and 1 M K-Na tartrate), then heated for 10 min at 100°C.
Importantly, no signs of toxicity was observed in mice (n=6) that received cell mixture treated with MegaFasL for 6 weeks, no premature death occurred in the treated group and the corresponding histopathology of liver, spleen, lung, gut, kidney and inguinal lymph nodes in this group was no different from untreated animals.
Application of this procedure to BM flushes from four paretic mice showed 74±21% (±s.e.m). of CD19+ cells with the Raji phenotype, whereas similar analysis on BM flushes from four available non-paretic mice injected with a HPC/Raji cell mixture treated with MegaFasL had 2±0.35% (±s.e.m). of CD19+ cells.
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And for the mixture treated group with higher dosage, the similar effects were also observed and no difference between these two groups.
For K. marxianus-treated coffee waste, the mixture was treated with K. marxianus mutant strain NRRL Y-50798 (Hughes et al. 2013) in a 30-L fermentation at 30 °C for 3 days at 100 rpm.
Upon completion, the reaction mixture was treated with additional chloroform (20 mL) and washed with distilled water (4 × 20 mL).
The digested nuclei mixture was treated with Proteinase K (Sigma-Aldrich, Missouri) and DNA was extracted using careful phenol chloroform extraction and ethanol/salt precipitation.
The mixture was treated with water and extracted with ethyl acetate.
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