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It didn't take long for the mixture to start boiling, and we were careful to not let the chocolate burn on the sides of the pot.
Mr. Darrington's preferred method was to mix a pint of water with two pounds of sand and then use this sloppy mixture to start building from the ground up in successive layers, which he then sculptured with a knife or lollipop stick.
After the preheating at 40 °C, 0.9 mL of NaBH4 solution (0.3 mM) was rapidly injected into this mixture to start the reaction.
Then, 2.5 µl of substrate solution (100 mM Z-Gly-Leu) was added to the mixture to start the enzymatic reaction at 40 °C for 30 min.
After mixing surface modified GNS and pyrrole monomers in ethylene solution, ferric chloride (FeCl3) is added to the mixture to start the polymerization, and GNS/PPy composites are formed.
Then, a substrate mixture of 2.5 µl of substrate solution (100 mM Z-Gly-Leu), 5 µl of 200 mM potassium phosphate buffer (pH 7), and 36.94 µl distilled water was prepared and added to the enzyme mixture to start the enzymatic reaction at 40 °C for 30 min.
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50 μL of PNPG at increasing concentrations (0.63 2.0 mg/mL) was added to both sets of reaction mixtures to start the reaction.
250 μL of starch solution at increasing concentrations (0.30 5.0 mg/mL) was added to both sets of reaction mixtures to start the reaction.
Non-enzymatic assay mixture contained 600 μL buffer and 200 μL paraoxon as substrate, while enzymatic assay mixture contained enzyme to start the reaction.
It is known that CdSe crystals dissolve quickly in a HNO3 + HCl mixture to form starting reagents CdCl2 and H2SeO3 [12].
Thus, general advice for the analysis of rich mixtures of foliar VOCs is to start with a 5% phenyl-substituted (polar) column.
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